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The results of solvent viscosity to the catalytic rate constants with the hydrolysis of 1 by Zn-NagA are proven in Figures 4A and 4B making use of sucrose given that the micro-viscogen. The plot link alternatif naga26 of relative k
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The constructions with the compounds examined as substrates for NagA are introduced in Scheme 6. The substitution of sulfur for oxygen may be used along side really hard and smooth metals to probe the potential for steel-ligand conversation in the course of the activation of electrophilic reactions (24, 25).
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To the thio-amide substrates, the Cd-enzyme was the best enzyme and the Zn-enzyme slot naga26 was poorer. The catalytic constants with the Zn- and Cd-substituted sorts of NagA aid the summary which the carbonyl group with the substrate is polarized by a immediate interaction with the single divalent cation certain to the Mβ-posture during the Energetic internet site.